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cryopreserved primary human hepatocytes  (BioIVT Inc)

 
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    BioIVT Inc cryopreserved primary human hepatocytes
    Cryopreserved Primary Human Hepatocytes, supplied by BioIVT Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cryopreserved+primary+human+hepatocytes/cryopreserved+human+hepatocytes/pm40551718-112-3-17
    Average 90 stars, based on 1 article reviews
    cryopreserved primary human hepatocytes - by Bioz Stars, 2026-10
    90/100 stars

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    Incubation:

    Article Title: In vitro mechanistic study on mycophenolate mofetil drug interactions: effect of prednisone, cyclosporine, and others
    Article Snippet: Additionally, 4-trifluoromethyl-7-hydroxycoumarin (Product No. 36852) and its metabolite, 4-trifluoromethyl-7-hydroxycoumarin glucuronide (Product No. T792035), were obtained from Sigma-Aldrich and Shanghai ZZBIO Co., Ltd. (Shanghai, China), respectively. .. Cryopreserved primary human hepatocytes (Lot No. HVN), as well as plating and incubation media, were purchased from BioIVT (Westbury, NY, USA). .. Recombinant human embryonic kidney (HEK) 293 cells expressing human OATP1B1 (HEK293-OATP1B1, Product No. GM1102G), OATP1B3 (HEK293-OATP1B3, Product No. GM1106G), OAT1 (HEK293- OAT1, Product No. GM1103G), OAT3 (HEK293-OAT3, Product No. GM1104G), and HEK293 cells transfected with an empty vector (HEK293-MOCK, Product No. GM1101G) were supplied by GenoMembrane (Tokyo, Japan).

    Centrifugation:

    Article Title: Two chemoattenuated PfSPZ malaria vaccines induce sterile hepatic immunity
    Article Snippet: .. To create micropatterned co-cultures, cryopreserved primary human hepatocytes (Bioreclamation IVT, BGW lot, male, age 50) were pelleted by centrifugation and then seeded on collagen-micropatterned plates. ..



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    Validation of cAAV in primary human <t>hepatocytes</t> (A) Schematic of cAAV design and mechanism. Orthogonal attachment sites (attB∗, attP∗) are added to an attP AAV genome to facilitate Bxb1-mediated intramolecular recombination. The reaction produces a circular dsDNA attP cargo and an ITR by-product. (B) Design of a ssAAV dual luciferase circularization reporter to validate cAAV function. In linear native format, EF1A promoter expresses Firefly luciferase, and NanoLuc is not expressed. When circularized, EF1A promoter expresses NanoLuc in the circular episome and Firefly luciferase is not expressed in the ITR by-product. (C) Relative amounts of linear or circularized cAAV reporter in PHH on days 1 and 5 following Bxb1 mRNA transfection. (D) Absolute quantification of cAAV circularization frequency on day 5 following Bxb1 mRNA transfection in PHH. (E) I-PGI outcome with cAAV or scAAV cargo over a 6-day time course in PHH. Statistical significance was calculated using Student’s unpaired two tailed t test (ns indicates not significant, ∗ p < 0.05, ∗∗ p < 0.01). (F) The effect of staggering cargo circularization and I-PGI on seamless insertion junction outcome in PHH. Predose is Bxb1 mRNA transfected twice, on day 2 and on day 5. For E-F, I-PGI was performed at the PAH locus through a single transfection of synthetic atgRNA, nCas9-RT and Bxb1 mRNAs. For (D–F), data were collected using ddPCR, and values were calculated as described in . Data reflect the mean and standard deviation of three biological replicates.
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    Validation of cAAV in primary human <t>hepatocytes</t> (A) Schematic of cAAV design and mechanism. Orthogonal attachment sites (attB∗, attP∗) are added to an attP AAV genome to facilitate Bxb1-mediated intramolecular recombination. The reaction produces a circular dsDNA attP cargo and an ITR by-product. (B) Design of a ssAAV dual luciferase circularization reporter to validate cAAV function. In linear native format, EF1A promoter expresses Firefly luciferase, and NanoLuc is not expressed. When circularized, EF1A promoter expresses NanoLuc in the circular episome and Firefly luciferase is not expressed in the ITR by-product. (C) Relative amounts of linear or circularized cAAV reporter in PHH on days 1 and 5 following Bxb1 mRNA transfection. (D) Absolute quantification of cAAV circularization frequency on day 5 following Bxb1 mRNA transfection in PHH. (E) I-PGI outcome with cAAV or scAAV cargo over a 6-day time course in PHH. Statistical significance was calculated using Student’s unpaired two tailed t test (ns indicates not significant, ∗ p < 0.05, ∗∗ p < 0.01). (F) The effect of staggering cargo circularization and I-PGI on seamless insertion junction outcome in PHH. Predose is Bxb1 mRNA transfected twice, on day 2 and on day 5. For E-F, I-PGI was performed at the PAH locus through a single transfection of synthetic atgRNA, nCas9-RT and Bxb1 mRNAs. For (D–F), data were collected using ddPCR, and values were calculated as described in . Data reflect the mean and standard deviation of three biological replicates.
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    Validation of cAAV in primary human <t>hepatocytes</t> (A) Schematic of cAAV design and mechanism. Orthogonal attachment sites (attB∗, attP∗) are added to an attP AAV genome to facilitate Bxb1-mediated intramolecular recombination. The reaction produces a circular dsDNA attP cargo and an ITR by-product. (B) Design of a ssAAV dual luciferase circularization reporter to validate cAAV function. In linear native format, EF1A promoter expresses Firefly luciferase, and NanoLuc is not expressed. When circularized, EF1A promoter expresses NanoLuc in the circular episome and Firefly luciferase is not expressed in the ITR by-product. (C) Relative amounts of linear or circularized cAAV reporter in PHH on days 1 and 5 following Bxb1 mRNA transfection. (D) Absolute quantification of cAAV circularization frequency on day 5 following Bxb1 mRNA transfection in PHH. (E) I-PGI outcome with cAAV or scAAV cargo over a 6-day time course in PHH. Statistical significance was calculated using Student’s unpaired two tailed t test (ns indicates not significant, ∗ p < 0.05, ∗∗ p < 0.01). (F) The effect of staggering cargo circularization and I-PGI on seamless insertion junction outcome in PHH. Predose is Bxb1 mRNA transfected twice, on day 2 and on day 5. For E-F, I-PGI was performed at the PAH locus through a single transfection of synthetic atgRNA, nCas9-RT and Bxb1 mRNAs. For (D–F), data were collected using ddPCR, and values were calculated as described in . Data reflect the mean and standard deviation of three biological replicates.
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    Validation of cAAV in primary human <t>hepatocytes</t> (A) Schematic of cAAV design and mechanism. Orthogonal attachment sites (attB∗, attP∗) are added to an attP AAV genome to facilitate Bxb1-mediated intramolecular recombination. The reaction produces a circular dsDNA attP cargo and an ITR by-product. (B) Design of a ssAAV dual luciferase circularization reporter to validate cAAV function. In linear native format, EF1A promoter expresses Firefly luciferase, and NanoLuc is not expressed. When circularized, EF1A promoter expresses NanoLuc in the circular episome and Firefly luciferase is not expressed in the ITR by-product. (C) Relative amounts of linear or circularized cAAV reporter in PHH on days 1 and 5 following Bxb1 mRNA transfection. (D) Absolute quantification of cAAV circularization frequency on day 5 following Bxb1 mRNA transfection in PHH. (E) I-PGI outcome with cAAV or scAAV cargo over a 6-day time course in PHH. Statistical significance was calculated using Student’s unpaired two tailed t test (ns indicates not significant, ∗ p < 0.05, ∗∗ p < 0.01). (F) The effect of staggering cargo circularization and I-PGI on seamless insertion junction outcome in PHH. Predose is Bxb1 mRNA transfected twice, on day 2 and on day 5. For E-F, I-PGI was performed at the PAH locus through a single transfection of synthetic atgRNA, nCas9-RT and Bxb1 mRNAs. For (D–F), data were collected using ddPCR, and values were calculated as described in . Data reflect the mean and standard deviation of three biological replicates.
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    Validation of cAAV in primary human <t>hepatocytes</t> (A) Schematic of cAAV design and mechanism. Orthogonal attachment sites (attB∗, attP∗) are added to an attP AAV genome to facilitate Bxb1-mediated intramolecular recombination. The reaction produces a circular dsDNA attP cargo and an ITR by-product. (B) Design of a ssAAV dual luciferase circularization reporter to validate cAAV function. In linear native format, EF1A promoter expresses Firefly luciferase, and NanoLuc is not expressed. When circularized, EF1A promoter expresses NanoLuc in the circular episome and Firefly luciferase is not expressed in the ITR by-product. (C) Relative amounts of linear or circularized cAAV reporter in PHH on days 1 and 5 following Bxb1 mRNA transfection. (D) Absolute quantification of cAAV circularization frequency on day 5 following Bxb1 mRNA transfection in PHH. (E) I-PGI outcome with cAAV or scAAV cargo over a 6-day time course in PHH. Statistical significance was calculated using Student’s unpaired two tailed t test (ns indicates not significant, ∗ p < 0.05, ∗∗ p < 0.01). (F) The effect of staggering cargo circularization and I-PGI on seamless insertion junction outcome in PHH. Predose is Bxb1 mRNA transfected twice, on day 2 and on day 5. For E-F, I-PGI was performed at the PAH locus through a single transfection of synthetic atgRNA, nCas9-RT and Bxb1 mRNAs. For (D–F), data were collected using ddPCR, and values were calculated as described in . Data reflect the mean and standard deviation of three biological replicates.
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    Validation of cAAV in primary human <t>hepatocytes</t> (A) Schematic of cAAV design and mechanism. Orthogonal attachment sites (attB∗, attP∗) are added to an attP AAV genome to facilitate Bxb1-mediated intramolecular recombination. The reaction produces a circular dsDNA attP cargo and an ITR by-product. (B) Design of a ssAAV dual luciferase circularization reporter to validate cAAV function. In linear native format, EF1A promoter expresses Firefly luciferase, and NanoLuc is not expressed. When circularized, EF1A promoter expresses NanoLuc in the circular episome and Firefly luciferase is not expressed in the ITR by-product. (C) Relative amounts of linear or circularized cAAV reporter in PHH on days 1 and 5 following Bxb1 mRNA transfection. (D) Absolute quantification of cAAV circularization frequency on day 5 following Bxb1 mRNA transfection in PHH. (E) I-PGI outcome with cAAV or scAAV cargo over a 6-day time course in PHH. Statistical significance was calculated using Student’s unpaired two tailed t test (ns indicates not significant, ∗ p < 0.05, ∗∗ p < 0.01). (F) The effect of staggering cargo circularization and I-PGI on seamless insertion junction outcome in PHH. Predose is Bxb1 mRNA transfected twice, on day 2 and on day 5. For E-F, I-PGI was performed at the PAH locus through a single transfection of synthetic atgRNA, nCas9-RT and Bxb1 mRNAs. For (D–F), data were collected using ddPCR, and values were calculated as described in . Data reflect the mean and standard deviation of three biological replicates.
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    cryopreserved, plateable, primary human hepatocytes  (Thermo Fisher)
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    Validation of cAAV in primary human <t>hepatocytes</t> (A) Schematic of cAAV design and mechanism. Orthogonal attachment sites (attB∗, attP∗) are added to an attP AAV genome to facilitate Bxb1-mediated intramolecular recombination. The reaction produces a circular dsDNA attP cargo and an ITR by-product. (B) Design of a ssAAV dual luciferase circularization reporter to validate cAAV function. In linear native format, EF1A promoter expresses Firefly luciferase, and NanoLuc is not expressed. When circularized, EF1A promoter expresses NanoLuc in the circular episome and Firefly luciferase is not expressed in the ITR by-product. (C) Relative amounts of linear or circularized cAAV reporter in PHH on days 1 and 5 following Bxb1 mRNA transfection. (D) Absolute quantification of cAAV circularization frequency on day 5 following Bxb1 mRNA transfection in PHH. (E) I-PGI outcome with cAAV or scAAV cargo over a 6-day time course in PHH. Statistical significance was calculated using Student’s unpaired two tailed t test (ns indicates not significant, ∗ p < 0.05, ∗∗ p < 0.01). (F) The effect of staggering cargo circularization and I-PGI on seamless insertion junction outcome in PHH. Predose is Bxb1 mRNA transfected twice, on day 2 and on day 5. For E-F, I-PGI was performed at the PAH locus through a single transfection of synthetic atgRNA, nCas9-RT and Bxb1 mRNAs. For (D–F), data were collected using ddPCR, and values were calculated as described in . Data reflect the mean and standard deviation of three biological replicates.
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    Validation of cAAV in primary human hepatocytes (A) Schematic of cAAV design and mechanism. Orthogonal attachment sites (attB∗, attP∗) are added to an attP AAV genome to facilitate Bxb1-mediated intramolecular recombination. The reaction produces a circular dsDNA attP cargo and an ITR by-product. (B) Design of a ssAAV dual luciferase circularization reporter to validate cAAV function. In linear native format, EF1A promoter expresses Firefly luciferase, and NanoLuc is not expressed. When circularized, EF1A promoter expresses NanoLuc in the circular episome and Firefly luciferase is not expressed in the ITR by-product. (C) Relative amounts of linear or circularized cAAV reporter in PHH on days 1 and 5 following Bxb1 mRNA transfection. (D) Absolute quantification of cAAV circularization frequency on day 5 following Bxb1 mRNA transfection in PHH. (E) I-PGI outcome with cAAV or scAAV cargo over a 6-day time course in PHH. Statistical significance was calculated using Student’s unpaired two tailed t test (ns indicates not significant, ∗ p < 0.05, ∗∗ p < 0.01). (F) The effect of staggering cargo circularization and I-PGI on seamless insertion junction outcome in PHH. Predose is Bxb1 mRNA transfected twice, on day 2 and on day 5. For E-F, I-PGI was performed at the PAH locus through a single transfection of synthetic atgRNA, nCas9-RT and Bxb1 mRNAs. For (D–F), data were collected using ddPCR, and values were calculated as described in . Data reflect the mean and standard deviation of three biological replicates.

    Journal: Molecular Therapy. Methods & Clinical Development

    Article Title: Development of circular AAV cargos for targeted seamless insertion with large serine integrases

    doi: 10.1016/j.omtm.2025.101490

    Figure Lengend Snippet: Validation of cAAV in primary human hepatocytes (A) Schematic of cAAV design and mechanism. Orthogonal attachment sites (attB∗, attP∗) are added to an attP AAV genome to facilitate Bxb1-mediated intramolecular recombination. The reaction produces a circular dsDNA attP cargo and an ITR by-product. (B) Design of a ssAAV dual luciferase circularization reporter to validate cAAV function. In linear native format, EF1A promoter expresses Firefly luciferase, and NanoLuc is not expressed. When circularized, EF1A promoter expresses NanoLuc in the circular episome and Firefly luciferase is not expressed in the ITR by-product. (C) Relative amounts of linear or circularized cAAV reporter in PHH on days 1 and 5 following Bxb1 mRNA transfection. (D) Absolute quantification of cAAV circularization frequency on day 5 following Bxb1 mRNA transfection in PHH. (E) I-PGI outcome with cAAV or scAAV cargo over a 6-day time course in PHH. Statistical significance was calculated using Student’s unpaired two tailed t test (ns indicates not significant, ∗ p < 0.05, ∗∗ p < 0.01). (F) The effect of staggering cargo circularization and I-PGI on seamless insertion junction outcome in PHH. Predose is Bxb1 mRNA transfected twice, on day 2 and on day 5. For E-F, I-PGI was performed at the PAH locus through a single transfection of synthetic atgRNA, nCas9-RT and Bxb1 mRNAs. For (D–F), data were collected using ddPCR, and values were calculated as described in . Data reflect the mean and standard deviation of three biological replicates.

    Article Snippet: Cryopreserved primary human hepatocytes (Thermo Fisher) were recovered in Cryopreserved Hepatocyte Recovery Media (Gibco CM7000) and plated at 37–42k cells per well.

    Techniques: Biomarker Discovery, Luciferase, Transfection, Quantitative Proteomics, Two Tailed Test, Standard Deviation